Plasmid Preparation:Article Title: Expression of the genes encoding the CasK/R two-component system and the DesA desaturase during Bacillus cereus cold adaptation.
Article Snippet: .. Strain Relevant genotype Reference or plasmid and characteristicsa or source Strains B. cereus ATCC 14579 WT ATCC 14579, wild type ATCC, USA B. cereus casKR WT bc2216-bc2217, Kmr Diomandé et al. (2014) E. coli TG1 (lac-proAB) supEthi hsd-5 (F′traD36 proA+proB+lacIqlacZ M15), general purpose cloning host Laboratory collection E. coli ET12567 F−dam-13::Tn9 dcm-6 hsdMhsdR recF143 zjj-202: :Tn10 galK2 galT22 ara14 pacY1 xyl-5 leuB6 thi-1, for generation of unmethylated DNA Laboratory collection E. coli BL21 Star(DE3) F−ompT hsdSB (rB−, mB−) gal dcm rne131 (DE3) Invitrogen Plasmids pHT304-casK/R′Z 376 bp region upstream from casK/R start codon inserted between HindIII and BamHI sites of pHT304-18′Z This study pHT304-rpiR′Z 329 bp region upstream from rpiR start codon inserted between HindIII and BamHI sites of pHT304-18′Z This study pHT304-desA′Z 634 bp region upstream from desA start codon inserted between HindIII and BamHI sites of pHT304-18′Z This study pHT304-18′Z ApR and EmR cloning plasmid; lacZ reporter gene Agaisse and Lereclus (1994) pET101/D-TOPO ApR cloning and expression plasmid Invitrogen aKm, kanamycin; Ap, ampicillin; Em, erythromycin. ..
Cloning:Article Title: Expression of the genes encoding the CasK/R two-component system and the DesA desaturase during Bacillus cereus cold adaptation.
Article Snippet: .. Strain Relevant genotype Reference or plasmid and characteristicsa or source Strains B. cereus ATCC 14579 WT ATCC 14579, wild type ATCC, USA B. cereus casKR WT bc2216-bc2217, Kmr Diomandé et al. (2014) E. coli TG1 (lac-proAB) supEthi hsd-5 (F′traD36 proA+proB+lacIqlacZ M15), general purpose cloning host Laboratory collection E. coli ET12567 F−dam-13::Tn9 dcm-6 hsdMhsdR recF143 zjj-202: :Tn10 galK2 galT22 ara14 pacY1 xyl-5 leuB6 thi-1, for generation of unmethylated DNA Laboratory collection E. coli BL21 Star(DE3) F−ompT hsdSB (rB−, mB−) gal dcm rne131 (DE3) Invitrogen Plasmids pHT304-casK/R′Z 376 bp region upstream from casK/R start codon inserted between HindIII and BamHI sites of pHT304-18′Z This study pHT304-rpiR′Z 329 bp region upstream from rpiR start codon inserted between HindIII and BamHI sites of pHT304-18′Z This study pHT304-desA′Z 634 bp region upstream from desA start codon inserted between HindIII and BamHI sites of pHT304-18′Z This study pHT304-18′Z ApR and EmR cloning plasmid; lacZ reporter gene Agaisse and Lereclus (1994) pET101/D-TOPO ApR cloning and expression plasmid Invitrogen aKm, kanamycin; Ap, ampicillin; Em, erythromycin. ..
Expressing:Article Title: Expression of the genes encoding the CasK/R two-component system and the DesA desaturase during Bacillus cereus cold adaptation.
Article Snippet: .. Strain Relevant genotype Reference or plasmid and characteristicsa or source Strains B. cereus ATCC 14579 WT ATCC 14579, wild type ATCC, USA B. cereus casKR WT bc2216-bc2217, Kmr Diomandé et al. (2014) E. coli TG1 (lac-proAB) supEthi hsd-5 (F′traD36 proA+proB+lacIqlacZ M15), general purpose cloning host Laboratory collection E. coli ET12567 F−dam-13::Tn9 dcm-6 hsdMhsdR recF143 zjj-202: :Tn10 galK2 galT22 ara14 pacY1 xyl-5 leuB6 thi-1, for generation of unmethylated DNA Laboratory collection E. coli BL21 Star(DE3) F−ompT hsdSB (rB−, mB−) gal dcm rne131 (DE3) Invitrogen Plasmids pHT304-casK/R′Z 376 bp region upstream from casK/R start codon inserted between HindIII and BamHI sites of pHT304-18′Z This study pHT304-rpiR′Z 329 bp region upstream from rpiR start codon inserted between HindIII and BamHI sites of pHT304-18′Z This study pHT304-desA′Z 634 bp region upstream from desA start codon inserted between HindIII and BamHI sites of pHT304-18′Z This study pHT304-18′Z ApR and EmR cloning plasmid; lacZ reporter gene Agaisse and Lereclus (1994) pET101/D-TOPO ApR cloning and expression plasmid Invitrogen aKm, kanamycin; Ap, ampicillin; Em, erythromycin. ..
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